Explore restriction digestion and DNA gel electrophoresis without the time needed to perform the digestion. The Analysis of Precut Lambda DNA Kit demonstrates the basic procedures and principles of DNA gel electrophoresis, including agarose gel casting, sample loading, size-based separation of DNA fragments, DNA staining, and graphic analysis. This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize DNA fragments.
- Save time — use precut lambda DNA
- Fast results — separate the fragments in as little as 10 minutes
- Optional sizing activity — guide students through the construction of a standard curve using their own gel data, then challenge them to apply their math skills and use their standard curve to estimate the molecular weights of the fragments generated by different restriction enzymes
- Finish all activities in one to two 45-minute class periods
- Consumable refill kit includes DNA digests, uncut lambda DNA, Fast Blast DNA stain, and more; for educational use only
This product has met the following criteria to qualify for the following awards: